Volume 2 · Issue 6 (2025)
Network Pharmacological Examination of Yujin's Efficacy in Stroke Treatment
Dong Yao
Beijing Anzhen Nanchong Hospital, Capital Medical University & Nanchong Central Hospital, Nanchong, China
Abstract: This study aimed to get to the bottom of the bioactive components in Yujin that pack a punch against stroke and the intricate ways they work their magic. We put the Traditional Chinese Medicine systems pharmacology platform (TCMSP) to work to filter out the key Yujin components and their target proteins. Meanwhile, we rounded up stroke-related targets from a lineup of databases including GeneCards, OMIM, DrugBank, and TTD. By mapping these elements with Venn diagrams, we zeroed in on the common ground. The STRING database helped us weave together a protein-protein interaction (PPI) network. Taking it a step further, we used Cytoscape 3.8.2 to build a comprehensive network linking active components, targets, and pathways. To get the full picture, we dove into Gene Ontology analysis—covering biological processes, molecular functions, and cellular components—through the DAVID database. Finally, to connect the dots on how Yujin gets the job done, we ran a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Yujin boasts three bioactive components that pack a punch against stroke, while simultaneously interacting with twelve crucial target proteins. Our Gene Ontology analysis revealed that Yujin’s impact on stroke management touches upon a whopping 1458 biological processes, 38 cellular components, and 143 molecular functions. When we dove deeper with KEGG pathway enrichment analysis, we found that Yujin works its therapeutic magic through several key pathways-think lipid metabolism and atherosclerosis, platinum drug resistance, toxoplasmosis, and more. The beauty of Yujin lies in its multi-target, multi-pathway approach, which makes it a valuable player in both preventing and treating stroke. The mechanisms we’ve uncovered in this study could pave the way for future experimental research and clinical applications down the road.
Keywords: Network pharmacology; Stroke, Mechanism; Yujin
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